Table 2. Affinities for Interactions between LSD1 and Linear Monosubstituted Analogues of H3_1-11 (4) Determined by an SPR Biosensor Assay.

N	sequencea	Xb	KD (μM)
4	ARTMQ TARKS T-OH		140
9	ARTKQ TARKS T-NH2		>100
11	PRTMQ TARKS T-NH2		10
12	XRTMQ TARKS T-NH2	Tle	nic
13	AXTMQ TARKS T-NH2	Cit	ni
14	ARTMQ TAXKS T-NH2	Cit	ni
15	ARTXQ TARKS T-NH2	Nle	>200
16	ARTXQ TARKS T-NH2	Aoc	>200
17	ARTXQ TARKS T-NH2	homo-Phe	>200

^aAll peptides had a N-terminal amine.

^bSubstitutions at X: Tle: l-tert-leucine, Cit: citrulline, Nle: norleucine, Aoc: 2-S-amino octanoic acid, homo-Phe: l-homophenylalanine.

^cni: no interaction observed.