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Functional validation of α7 nAChR constructs with single non-native cysteine. Response to agonist in Xenopus oocytes injected with RNA for the indicated constructs is similar to the wild-type α7 nAChR (bold line). Only V444C is significantly different from wild type (p=0.5) based on the extra sum-of-squares F test. Error bars indicate standard error.
