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. 2020 Jan 19;8(1):e000210. doi: 10.1136/jitc-2019-000210

Figure 7.

Figure 7

In a human xenograft model, iaIL-12 enhanced treatment efficacy without increasing systemic IL-12 exposure. (A) Human T cells were cotransduced to express the E7 TCR and the IL-12 constructs indicated. The transduced T cells were administered by intraperitoneal injection to immunodeficient mice bearing 12-day, established, CaSki, subcutaneous tumors. isIL-12 served as a positive control for systemic IL-12 exposure. F5M (MART-1-specific TCR) served as a negative TCR control targeting an irrelevant antigen. Serial tumor measurements were obtained. N=5 mice per group. Error bars show the standard error of the mean. P values represent comparisons between iaIL-12 and iGFP with a matched-pairs, repeated-measures two-way ANOVA test. **P<0.01. (B) Serum was collected from treated mice 14 days after treatment. The IL-12 concentration was measured with a multiplex cytokine kit. Values for individual mice are plotted. ANOVA, analysis of variance; E7TCR, T cell receptor targeting human papilloma virus-16 E7; iaIL-12, inducible anchored interleukin-12; iGFP, inducible green fluorescent protein; IL-12, interleukin-12; isIL-12, inducible secreted interleukin-12; TCR, T cell receptor.