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. 2020 Mar 4;15:6. doi: 10.1186/s13062-020-0258-5

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Comparison of MLSA and species tree. a Maximum-likelihood phylogenetic tree of 116 BCC genomes based on concatenated amino acid alignments of 1005 single-copy orthologous genes (274,980 AA) and rooted at node pointed out by MLSA phylogeny. Node support values were based on the Shimodaira-Hasegawa test. b Maximum-likelihood phylogenetic tree of the concatenated nucleotide sequences (2771 bp) from the seven housekeeping gene fragments [atpD (443 bp), gltB (400 bp), gyrB (454 bp), recA (393 bp), lepA (395 bp), phaC (385 bp) and trpB (301 bp)]. B. pseudomallei K96243, B. oklahomensis C6786^T and B. glumae LMG 2196^T were chosen as outgroups. Other display settings in (a) and (b) are the same as in Fig. 1