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. 2020 Mar 4;16:75. doi: 10.1186/s12917-020-02298-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — West blot results of samples. a showed the results of western blot about Occludin and Claudin-1. After treated for 24 h, various concentrations of betaine (0-2 mmol/L) improve the expression Occludin and Claudin-1 in IPEC-J2 cells. c confirms that LPS down-regulate the protein levels of Occludin and Claudin-1, while betaine totally attenuated the down-regulation and recovered to a normal level. b and d were the statistical graphs of the density ratios of different proteins to β-actin calculated by Image J and analyzed by Graphpad Prism6. All the results were repeated four times (n = 4, *P < 0.05, **P < 0.01 compared with control)