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. 2020 Mar 4;16:75. doi: 10.1186/s12917-020-02298-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — RT-PCR results of samples. a confirmed that betaine(2 mmol/L, treated for 4 h)can recover the down-regulation of Occludin induced by LPS(1 μg/mL), and enhanced the mRNA expression of Occludin. b proved that LPS induced inflammation while co-treatment of betaine and LPS make the IL-6expression drop to normal levels. Besides, betaine alone will lower the IL-6 expression. All the results were repeated three times (n = 3, *P < 0.05, ** P < 0.01, ***P < 0.001 compared with control)