Table 1.
Primer sequences of Kompetitive allele-specific PCR (KASP) markers specific for 14 rye chromosome arms
| KASP marker | Location | Primer sequences (5′-3′) |
|---|---|---|
| SWK5282 -F | 1RS | gaaggtgaccaagttcatgctGAGCTGATTTCCATGTA |
| SWK5282 -H | gaaggtcggagtcaacggattGAGCTGATTTCCATGTC | |
| SWK5282 -C | TACCAAGTCCTGAACCA | |
| SWK23822-F | 1RL | gaaggtgaccaagttcatgctTATGGGAATTTATGGCCGCA |
| SWK23822-H | gaaggtcggagtcaacggattTATGGGAATTTATGGCCGCG | |
| SWK23822-C | CCCGGAAAAGCTCCTTTT | |
| SWK621-F | 2RS | gaaggtgaccaagttcatgctGAGGAAGCTCCATCAATCTG |
| SWK621-H | gaaggtcggagtcaacggattGAGGAAGCTCCATCAATCTT | |
| SWK621-C | CACCGAATCAATCATGCAAC | |
| SWK252224-F | 2RL | gaaggtgaccaagttcatgctTCAACACCAAGAGAAGGGAAC |
| SWK252224-H | gaaggtcggagtcaacggattTCAACACCAAGAGAAGGGAAA | |
| SWK252224-C | CAGATGCATGTAGGTAGCGC | |
| SWK28002-F | 3RS | gaaggtgaccaagttcatgctCGGACAATGCACGATCGA |
| SWK28002-H | gaaggtcggagtcaacggattCGGACAATGCACGATCGG | |
| SWK28002-C | CGCACGCACATCAACACG | |
| SWK15063-F | 3RL | gaaggtgaccaagttcatgctCGAAAGTATGGGCTGCATTTT |
| SWK15063-H | gaaggtcggagtcaacggattCGAAAGTATGGGCTGCATTTC | |
| SWK15063-C | CCGACCCGTTCAGCCATT | |
| SWK30487-F | 4RS | gaaggtgaccaagttcatgctGGGTCGAGGTAGGTGAGG |
| SWK30487-H | gaaggtcggagtcaacggattGGGTCGAGGTAGGTGAGC | |
| SWK30487-C | GCTGACGGCACAATCAAC | |
| SWK61253-F | 4RL | gaaggtgaccaagttcatgctTGAAGTACTCAGCATTCAGC |
| SWK61253-H | gaaggtcggagtcaacggattTGAAGTACTCAGCATTCAGT | |
| SWK61253-C | GTTCTCTTGTTCACACTCCAGT | |
| SWK190654-F | 5RS | gaaggtgaccaagttcatgctAGGCCAAGAGAAGAGTGAAGAC |
| SWK190654-H | gaaggtcggagtcaacggattAGGCCAAGAGAAGAGTGAAGAT | |
| SWK190654-C | TAACTACCGGCTGCCCTTTT | |
| SWK37355-F | 5RL | gaaggtgaccaagttcatgctTTCTGGTCCTAACGCTGAA |
| SWK37355-H | gaaggtcggagtcaacggattTTCTGGTCCTAACGCTGAG | |
| SWK37355-C | CTGCATGCAATTCAAGACAGA | |
| SWK38534-F | 6RS | gaaggtgaccaagttcatgctTGAATCTCAACCATGCCCTT |
| SWK38534-H | gaaggtcggagtcaacggattTGAATCTCAACCATGCCCTC | |
| SWK38534-C | CCTTGACTGTGTGGCCGATT | |
| SWK26615-F | 6RL | gaaggtgaccaagttcatgctCACTTTAACTTGGCGTTGGAG |
| SWK26615-H | gaaggtcggagtcaacggattCACTTTAACTTGGCGTTGGAC | |
| SWK26615-C | CTGAACTGGCCATTTGCA | |
| SWK31799-F | 7RS | gaaggtgaccaagttcatgctACCTGAATATTGGGCGCA |
| SWK31799-H | gaaggtcggagtcaacggattACCTGAATATTGGGCGCC | |
| SWK31799-C | TCACAGATCAACCTAGCCTCC | |
| SWK13002-F | 7RL | gaaggtgaccaagttcatgctATGCTTCTGCTGGTCTCTT |
| SWK13002-H | gaaggtcggagtcaacggattATGCTTCTGCTGGTCTCTC | |
| SWK13002-C | CCAATACAGGAGTAGATCGAC |