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. 2020 Mar 5;18:36. doi: 10.1186/s12964-019-0507-3

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Identification and sorting of BCSCs using flow cytometry from the human breast cell lines. (a) Sorting of BCSCs identified as either ALDH^bright or CD44⁺/CD24⁻/ESA⁺ cells from MDA-MB-231 and UACC-812 cell lines (b) Sorted ALDH^bright as well as CD44⁺/CD24⁻/ESA⁺ BCSCs expressed greater levels of ALDH1A1 mRNA than in non-BCSCs (c) Mammosphere formation from sorted BSCSs and non-BSCSs