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. 2020 Mar 4;13:32. doi: 10.1186/s12920-020-0671-8

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Development of a specific and sensitive NGS panel for FLT3-ITD detection and disease monitoring. a Serial dilution results for MV4–11 cell line (30 bp ITD in FLT3). The x-axis is allele fraction and y-axis is the number of ITD supporting reads / 10 M sequencing reads. b Nine leukemia samples were analyzed with varying ITD sizes (right y-axis, grey bars) and allele fraction (left y-axis, blue bars). Subjects are across the x-axis, with D = diagnosis, EOI = end of induction, and R = relapse sample