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. 2020 Mar 4;16:76. doi: 10.1186/s12917-020-02297-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Optimization and detection limit of the RPA-LFD method. (a) Image showing the LFD results of RPA amplification under different temperatures. The temperatures under which the RPA reactions were performed are indicated on top of each dipstick. The amplification template was V. alginolyticus. The NTC dipstick is the no-template control that was performed at 40 °C. (b) Image showing the LFD results of RPA amplification with different reaction times. The times for which the RPA reactions were performed are indicated on top of each dipstick. The amplification template was V. alginolyticus. The NTC dipstick is the no-template control that was performed for 45 min. (c) Image showing the LFD results of RPA amplification with different amounts of V. alginolyticus. The amounts tested are indicated on top of each dipstick. The NTC dipstick is the no-template control. The reactions were performed at 40 °C for 25 min. (d) Image showing the LFD results of RPA amplification with different amounts of purified genomic DNA of V. alginolyticus. The amounts of DNA tested are indicated on top of each dipstick. The NTC dipstick is the no-template control. The reactions were performed at 40 °C for 25 min. (e) Image showing the RPA-LFD test results for shrimp samples spiked with V. alginolyticus. The spiked amounts are indicated on top of each dipstick. The NC dipstick is the control of the shrimp sample without spiking. The NTC dipstick is the no-template control. The reactions were performed at 40 °C for 25 min. The positions of the control and test lines are indicated on the right of the images