Table 1.

Primers and probes used in this study

Name	Sequence (5′ to 3′)	Amplicon size
F1	5′-GAGCCCATTTTCTACTGACTCTAACACTGAC	252
F2	5′-AGCCCATTTTCTACTGACTCTAACACTGAC	251
F3	5′-GCCCATTTTCTACTGACTCTAACACTGACGT	250
F4	5′-CCCATTTTCTACTGACTCTAACACTGACGT	249
F5	5′-TTCTACTGACTCTAACACTGACGTTGAAGAAACC	243
F6	5′-TCTACTGACTCTAACACTGACGTTGAAGAA	242
F7	5′-ACTGACTCTAACACTGACGTTGAAGAAACCG	239
F8	5′-CTGACTCTAACACTGACGTTGAAGAAACCG	238
F9	5′-GAGATAGTGACCGATACTACTGCTGATCTTGAG	152
F10	5′-AGATAGTGACCGATACTACTGCTGATCTTG	151
F11	5′-AGATAGTGACCGATACTACTGCTGATCTTGA	151
F12	5′-ATAGTGACCGATACTACTGCTGATCTTGAG	149
R	5′-Biotin -GTTCGTGAATAACAATACGCAAACAGGAAG	/
Probe 1	5′-FITC-AAGCGCCAGCAGTGGAGTTAGAAGCGAGCG [THF]TACACCACCAACAGA-DDC	/
Probe 2	5′-FITC-TCAAGTAGAGCCGACTAAAACTCAGCCGAA [THF]CCAGCATCTAACACG-DDC	/
AlgF1	5′-TCAGAGAAAGTTGAGCTAACGATT	568
AlgR1	5′-CATCGTCGCCTGAAGTCGCTGT
16S rRNA-F	5′-AGAGTTTGATCCTGGCTCAG	/
16S rRNA-R	5′-TACGGTTACCTTGTTACGACTT

(F: forward primer. R: reverse primer)