Fig. 2.

Custom panel targeted RNA sequencing of treated and untreated mice. Bone marrow-derived leukemic blast populations of controls and treated mice sacrificed at d10, d13 and d15 (two mice per group and time point) were analyzed for changes in gene expression of 177 genes involved in BCR, PI3K/AKT and CK2 downstream signaling. a Principal component analysis blot of control animals (blue) and CX-4945-treated animals (red) demonstrating no distinct clustering of subgroups. b Hierarchical clustering of all genes and all animals throughout all observation time points. c Fold changes of apoptosis-related genes within the panel after CX-4945 treatment compared to time-matched controls. No significant regulation (fold change > 2 or < − 2) was observed. Red boxes indicate genes with consistent gene expression changes throughout all time points