Figure 2.

HIV-1 RT activity by TCI and its components measured by the filter binding assay. P/T (1 μM) and HIV RT (50 nM) were incubated with dTTP or first dTTP analogue and incubation for 45 min at 37 °C. Next, second dTTP analogue was added to samples 4 and 5, and all samples were further incubated for 30 min at 37 °C followed by the addition of the dNTP mixture (100 mM dATP, 100 mM dTTP, 100 mM dCTP, and 10 mM [³H]dGTP and incubation for 45 min at 37 °C. All reactions were quenched by the addition of EDTA (0.5 M) and analyzed by filter binding assay as described in the Supporting Information. The data of three independent experiments with single standard deviations are presented.