Figure 2.

Detection and identification of HSP7C. HeLa cell lysates were used as the source of antigens, incubated with antibodies from the serum samples and analyzed using western blotting. (A) Positive ~70-kDa bands were present in 8/12 of the cell lysates treated with serum samples from patients with KD. (B and C) The 70-kDa band was not present in the (B) FC and (C) HC groups. (D and E) The amino acid sequence of the 70-kDa target band was identified as HSP7C using mass spectrometry. FC, febrile control; HC, healthy control; HSP7C, heat shock cognate 71 kDa protein; KD, Kawasaki disease.