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. 2020 Feb 10;43(4):1067–1080. doi: 10.3892/or.2020.7495

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Copyright: © Hao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — SFN-induced cytotoxicity in colon cancer cells. (A) Structure of SFN, also known as 1-isothiocyanato-4-(methylsulfinyl)-butane (CAS, 4478-93-7; molecular formula, C6H11NOS2). (B) HT-29 and SW480 cells were incubated with 0, 1.25, 2.5, 5, 10, 20, 40, 80 and 160 µM SFN for 24 h. (C and D) An MTT assay was used to detect cell viability after treatment with various concentrations of SFN or for different time-points (24, 48 and 72 h). The statistical significance of the results was analyzed by two-way ANOVA. (E and F) Colon cancer cells (HT-29 and SW480) were cultured with various concentrations of SFN (0, 5, 10 and 20 µM). A representative image of colony formation from 3 independent experiments is presented. (G) The number of colonies of various groups presented in the graphs of parts E and F were quantified. The statistical significance of the results was analyzed by one-way ANOVA. The results are presented as the mean ± standard deviation of 3 independent experiments. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. SFN, sulforaphane; ANOVA, analysis of variance.