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. 2020 Feb 10;43(4):1067–1080. doi: 10.3892/or.2020.7495

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Copyright: © Hao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effect of Nrf2 on UGT1A expression induced by SFN. The Nrf2 gene was knocked down in SW480 and HT-29 cells. (A and B) The Nrf2 protein levels in HT-29 and SW480 cells were assessed by immunoblotting. (C) The expression levels of Nrf2 were quantified by ImageJ and illustrated by a bar legend. The statistical significance of the results was analyzed by Student's t-test. ****P<0.0001 vs. NC shRNA. (D) The transcriptional level of Nrf2 was quantified by RT-qPCR and illustrated by a bar legend. ****P<0.0001 vs. NC shRNA. The statistical significance of the results was analyzed by Student's t-test. (E and F) The expression levels of UGT1A in HT-29 and SW480 cells decreased in Nrf2-knockdown cells, and the induction effect of SFN on UGT1A expression decreased upon Nrf2-gene knockdown. (G and H) The UGT1A expression in various groups presented in the images of parts E and F was quantified by ImageJ. The statistical significance of the results was analyzed by two-way analysis of variance. *P<0.05, **P<0.01, ***P<0.001 vs. the SFN 0 µM group; ^##P<0.01, ^#####P<0.0001 vs. the NC shRNA group. The results are expressed as the mean ± standard deviation of 3 independent experiments. SFN, sulforaphane; Nrf2, nuclear factor, erythroid 2 like 2; NC, negative control; shRNA, short hairpin RNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.