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. 2020 Feb 20;21(4):1717–1726. doi: 10.3892/mmr.2020.10993

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Scheme showing breeding of PDHA1^flox/flox Ins-cre^+/− mice. PDHA1^flox/flox mice were mated with Ins-cre^+/− mice and then F2 PDHA1^flox/− Ins-cre^+/− mice generation was obtained. PDHA1^flox/flox Ins-cre^+/− mice were generated by crossing PDHA1^flox/− Ins-cre^+/− mice with PDHA1^flox/flox mice. Genotyping was performed on 4-week-old mice using PCR. PDHA1, pyruvate dehydrogenase E1-alpha subunit.