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. 2020 Feb 20;21(4):1890–1896. doi: 10.3892/mmr.2020.10992

Figure 1.

Figure 1.

Validation and characteristics of hsa_circ_0001380 in human peripheral blood mononuclear cells. (A) Pattern of hsa_circ_0001380 formation and design of divergent primers. Red arrows indicate the forward primer and green arrows indicate the reverse primer. (B) Melting curve analysis of hsa_circ_0001380, constructed via RT-qPCR. (C) Agarose gel electropherogram of the RT-qPCR product. Lanes 1, 2 and 3 correspond to the DNA marker, GAPDH and hsa_circ_0001380, respectively. (D) RT-qPCR analysis of hsa_circ_0001380 and GAPDH after treatment with (RNase R) or without RNase R (Mock). RT-qPCR, reverse transcription-quantitative polymerase chain reaction; F, forward; R, reverse.