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. 2020 Feb 13;21(4):1761–1770. doi: 10.3892/mmr.2020.10987

Figure 4.

Figure 4.

MALAT1 regulates the miR-17-5p/ABCA1 axis. (A) Relative expression level of miR-17-5p in macrophages with NC or MALAT1 siRNA transfection. (B) Relative expression level of ABCA1 in macrophages with NC or MALAT1 siRNA transfection. (C) Putative binding sites between MALAT1 and miR-17-5p, and the mutant sites in the mut-MALAT1 reporter vector. (D) Luciferase activity analysis of 293T cells co-transfected with wt-MALAT1 or mut-MALAT1, and NC mimics or miR-17-5p mimics. (E) Relative mRNA expression level of ABCA1 in macrophages transfected with NC mimics or miR-17-5p mimics. (F) Relative mRNA expression level of ABCA1 in macrophages transfected with NC inhibitor or miR-17-5p inhibitor. (G) Putative binding sites between miR-17-5p and ABCA1 3′UTR, and the mutant sites in mut-ABCA1 3′UTR reporter vector. (H) Luciferase activity analysis in 293T cells co-transfected with wt-ABCA1 or mut-ABCA1, and NC mimics or miR-17-5p mimics. Data are presented as the mean ± SD. **P<0.01, ***P<0.001 vs. NC group [(A and B) NC siRNA; (D) wt-MALAT1 + NC mimics; (F) NC inhibitor; (H) wt-ABCA1 + NC mimics]. wt, wild-type; mut, mutant; NC, negative control; miR, microRNA; ABCA1, ATP-binding cassette transporter A1; MALAT1, metastasis associated lung adenocarcinoma transcript 1; siRNA, small interfering RNA; 3′UTR, 3′-untranslated region.