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. 2020 Feb 18;21(4):1941–1949. doi: 10.3892/mmr.2020.10989

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — TET2 modulates the levels of 5-hmC in HaCaT cells. Protein levels of TET2 were (A) determined by western blotting and (B) quantified following transfection with three specific siRNAs. (C) mRNA expression levels of TET2 were analyzed by reverse transcription-quantitative PCR in HaCaT cell transfected with si-TET2 or si-NC. Protein levels of TET2 were (D) determined by western blotting and (E) quantified following transfection with TET2-overexpression plasmid or NC plasmid. (F) mRNA expression levels of TET2 following transfection with TET-2 overexpression plasmid or NC plasmid. (G) Dot blot analyses for 5-hmC were performed following TET2 knockdown or overexpression in HaCaT cells. Data are presented as the mean ± SD of three independent experiments performed in triplicate. **P<0.01, ***P<0.001 vs. NC. TET2, ten-eleven translocation-2; 5-hmC, 5-hydroxymethylcytosine; si/siRNA, small interfering RNA; NC, negative control.