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. 2020 Feb 18;21(4):1941–1949. doi: 10.3892/mmr.2020.10989

Figure 4.

Figure 4.

TET2 expression alters the expression of the cell cycle-associated protein P16INK4a in HaCaT cells. Cell cycle profile of HaCaT cells transfected with si-TET2 was (A) determined by flow cytometry and (B) quantified. Cell cycle profile of HaCaT cells transfected with TET2-overexpression plasmid was (C) determined by flow cytometry and (D) quantified. P16INK4a protein levels were (E) determined by western blotting and (F) quantified following TET2 knockdown in HaCaT cells. (G) P16INK4a mRNA levels following TET2 knockdown in HaCaT cells were determined by RT-qPCR. P16INK4a protein levels were (H) determined by flow cytometry and (I) quantified following TET2 overexpression in HaCaT cells. (J) P16INK4a mRNA levels following TET2 overexpression in HaCaT cells were determined by RT-qPCR. Data are presented as the mean ± SD of three independent experiments performed in triplicate. *P<0.05, **P<0.01 vs. NC group. TET2, ten-eleven translocation-2; P16INK4a, cyclin-dependent kinase inhibitor 2A; si, small interfering RNA; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; PI, propidium iodide; ns, not significant.