Figure 2.

Reduced in vitro class switch in B cells from P2KO vs. WT mice. B cells were isolated from the spleens of young and old WT and P2KO mice (6 mice/group) by magnetic sorting. B cells (10⁶/ml) were stimulated for 1–7 days with 1 μg/ml of LPS, then mRNA was extracted and qPCR performed to evaluate mRNA expression of E47 (A) and Pax5 (B) (at day 1), AID (C) (at day 5), and Prdm1 (Blimp-1) (D) (at day 4). Results show qPCR values (2^−ΔΔCt). At day 5, cells are harvested to evaluate frequencies of CD138+ cells by flow cytometry (F). At day 7, supernatants were collected to measure IgG3 secretion by ELISA (E). Mean comparisons between groups were performed by two-way ANOVA followed by Bonferroni's multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.