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Purification scheme of GPx from the cell lysate of L. plantarum.
| Total protein in mg | Total activity in U | Specific activity U/mg | Yield (%) | Purification n-fold | |
|---|---|---|---|---|---|
| 1-Crude extract | 2.01 | 1231.1 | 612.5 | 100 | – |
| 2-DEAE-Sephadex A-50 | 1.73 | 1164 | 672.8 | 94.5 | 1.1 |
| 3-DEAE-Sephadex A-50 | 1.42 | 1110 | 781.7 | 90.1 | 1.28 |
| 4-Sephacryl S-200 | 1.16 | 1023.2 | 882.1 | 83.1 | 1.44 |
| 5-DEAE-Sephadex A-50 | 0.79 | 978.3 | 1238.4 | 79.5 | 2 |
| 6-Sephacryl S-200 | 0.47 | 917 | 1951.1 | 74.5 | 3.19 |
| 7-Mono Q | 0.25 | 522 | 2088 | 42.4 | 3.41 |
