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. 2020 Feb 26;8:114. doi: 10.3389/fcell.2020.00114

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Copyright © 2020 Mashkaryan, Siddiqui, Popova, Cosacak, Bhattarai, Brandt, Govindarajan, Petzold, Reinhardt, Dahl, Lefort and Kizil.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) SOX2 and IL4R immunostaining on brain sections of 12 months old wild type mouse. (B) DAPI added to (A). (C) SOX2-positive cells do not express IL4R. (C′) IL4R fluorescence channel alone. (D) GFAP and IL4R immunostaining on brain sections of 12 months old wild type mouse. (E) DAPI added to (D). (F) GFAP-positive cells do not express IL4R. (F′) IL4R fluorescence channel alone. (G) Iba1 and IL4R immunostaining on brain sections of 12 months old wild type mouse. (H) DAPI added to (G). (I) Iba-positive cells express IL4R. (I′) IL4R fluorescence channel alone. n = 3 animals. Scale bars: 100 μm (A,B,D,E,G,H) and 25 μm elsewhere.