Skip to main content
. 2020 Jan 31;43(4):1221–1233. doi: 10.3892/or.2020.7486

Figure 2.

Figure 2.

Overexpression of miR-299-5p affects the expression of EMT markers and MMPs. (A) The relative expression of miR-299-5p in MDA-MB-231 and MCF-7 cells following transfection with miR-299-5p mimics was assessed by reverse transcription-quantitative PCR. **P<0.01. (B) Western blotting detected the protein expression of EMT markers (N-cadherin, E-cadherin and vimentin) and MMP-2/9 in MDA-MB-231 and MCF-7 cells following transfection with miR-299-5p mimics or inhibitor. β-actin was used as an internal control. (C) Quantification of protein expression by grayscale analysis. *P<0.05, **P<0.01. EMT, epithelial-mesenchymal transition; MMP, matrix metallopeptidase.