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. 2020 Jan 31;43(4):1221–1233. doi: 10.3892/or.2020.7486

Figure 3.

Figure 3.

miR-299-5p inhibits the invasion and migration of breast cancer cells in vitro and in vivo. (A) Migration assays of MDA-MB-231 and MCF-7 cells following transfection with miR-299-5p mimics or inhibitor. The number of migrating cells was quantified using at least five random fields for each membrane (magnification, ×200). *P<0.05, **P<0.01. (B) Invasion assays of MDA-MB-231 and MCF-7 cells following transfection with miR-299-5p mimics or inhibitor. The number of invading cells was quantified using at least five random fields (magnification, ×200). *P<0.05, **P<0.01. (C) Wound healing assays of MDA-MB-231 and MCF-7 cells following transfection with miR-299-5p mimics or inhibitor. Images of the cells were captured every 24 h, and the width of the remaining gap was measured. (D) Quantification of the wound healing assay. The distance of cell migration was used to quantify the migratory capacity. *P<0.05, **P<0.01. (E) Representative images of H&E-stained sections of pulmonary metastases in nude mice (n=10) injected with miR-299-5p-overexpressing MDA-MB-231 cells compared with those injected with MDA-MB-231 negative control cells. **P<0.01. Data are expressed as the mean ± standard error of mean. LV, lentiviral; ctrl, control; H&E, haematoxylin and eosin.