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. 2020 Jan 31;43(4):1221–1233. doi: 10.3892/or.2020.7486

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — STK39 is upregulated in breast cancer. (A) STK39 expression levels were measured by immunohistochemistry in human breast cancer and adjacent non-cancerous tissue samples (magnification, ×400). (B) The percentage of positive cells was counted to evaluate the expression of STK39. **P<0.01. (C) The relative expression of STK39 mRNA in normal breast epithelial cell lines and breast cancer cell lines was measured by reverse transcription-quantitative PCR, and the expression of STK39 was normalized to β-actin. *P<0.05, **P<0.01. (D) The expression of STK39 in normal breast epithelial cells and breast cancer cell lines was measured by western blotting. β-actin was used as an internal control. (E) Quantification of protein expression by grayscale analysis. **P<0.01. Data are expressed as the mean ± standard error of mean. STK39, serine/threonine kinase 39.