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. 2020 Jan 31;43(4):1221–1233. doi: 10.3892/or.2020.7486

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Knockdown of STK39 by siRNA transfection in breast cancer cells. (A) The relative expression of STK39 mRNA was measured by reverse transcription-quantitative PCR in MDA-MB-231 and MCF-7 cells after transfection with si-STK39, and the expression of STK39 was normalized to β-actin. **P<0.01. (B) Western blotting detected the expression of EMT markers (N-cadherin, E-cadherin and vimentin), MMP-2/9, and STK39 in MDA-MB-231 and MCF-7 cells after transfection with si-STK39 or si-ctrl. β-actin was used as an internal control. (C) Quantification of protein expression by grayscale analysis. **P<0.01. STK39, serine/threonine kinase 39; EMT, epithelial-mesenchymal transition; MMP, matrix metallopeptidase.