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. 2020 Feb 15;98(3):skaa053. doi: 10.1093/jas/skaa053

Table 2.

Analyzed chemical composition of the experimental diets of Exp. 1 and Exp. 2, g/kg DM, if not stated otherwise

Exp. 1 Exp. 2
BD1 BDSBM2 BDRSC3
Phytase: 04 750 1,500 3,000 04 1,500 04 1,500
Dry matter, g/kg 900 902 901 901 893 894 893 895
Ash 57 56 56 55 60 59 59 58
Ether extract 66 68 65 66 58 58 83 85
Crude protein 187 192 187 188 233 235 199 202
Crude fiber 23 27 28 27 21 21 35 33
Gross energy, MJ/kg DM 19.5 19.6 19.5 19.5 19.2 19.2 19.5 19.6
Calcium 7.0 7.0 6.9 6.8 5.7 5.4 6.8 6.4
Total phosphorus (P) 3.7 3.8 3.7 3.7 4.4 4.5 5.5 5.4
InsP6-P 2.4 2.5 2.4 2.5 2.9 3.0 3.9 3.8
InsP6, µmol/g DM 13.0 13.3 13.1 13.3 15.5 16.1 21.1 20.3
Ins(1,2,3,4,6)P5, µmol/g DM <LOQ5 <LOQ <LOQ <LOQ 0.3 0.3 0.2 0.2
Ins(1,2,3,4,5)P5, µmol/g DM 0.3 0.4 0.4 0.4 0.6 0.6 0.4 0.4
Ins(1,2,4,5,6)P5, µmol/g DM 0.9 0.9 0.9 0.9 1.1 1.1 0.8 0.7
myo-Inositol, µmol/g DM 1.6 1.7 1.6 1.7 1.7 1.7 1.1 1.7
Phytase activity (FTU/kg)6 <50 792 1,710 3,140 <50 1,530 <50 1,370
Indispensable amino acids
 Arg 11.7 11.8 11.7 11.7 15.7 15.6 12.1 12.1
 His 5.5 5.7 5.6 5.6 6.9 6.8 5.8 5.9
 Ile 7.7 7.9 7.6 7.6 10.2 10.0 7.9 7.9
 Leu 17.0 17.3 17.0 17.1 21.1 20.9 17.3 17.2
 Lys 10.4 10.3 10.2 10.4 12.8 12.7 10.5 10.5
 Met 3.0 3.0 3.0 3.0 3.6 3.6 3.5 3.5
 Phe 9.4 9.6 9.4 9.4 12.0 11.9 9.2 9.2
 Thr 7.6 7.8 7.6 7.7 9.4 9.4 8.2 8.2
 Trp 2.3 2.4 2.2 2.2 2.9 3.0 2.6 2.6
 Val 8.7 8.9 8.6 8.6 11.2 11.1 9.5 9.5
Dispensable amino acids
 Ala 10.2 10.4 10.2 10.3 12.3 12.1 10.4 10.4
 Asx7 19.0 19.6 19.0 19.1 25.4 25.2 17.9 17.9
 Cys 3.0 3.1 3.0 3.0 3.5 3.5 3.8 3.8
 Glx7 33.8 34.8 34.1 34.3 43.8 43.7 35.9 35.6
 Gly 7.9 8.1 7.9 8.0 9.9 9.8 8.8 8.8
 Pro 10.8 10.5 10.7 10.6 13.9 13.9 12.6 12.6
 Ser 9.6 9.9 9.7 9.9 12.2 12.2 9.6 9.6
 Tyr 6.6 6.8 6.6 6.6 8.3 8.2 6.6 6.6

1Corn–soybean meal (SBM)-based basal diet of experiment 1 (Exp. 1.).

2Corn–SBM-based basal diet of experiment 2 (Exp. 2); analyzed concentration of nutrients in SBM, in % on dry matter (DM) basis: P, 0.75, Ca, 0.51; CP, 51.2; EE, 2.6; NDF, 17.3; ADF, 6.9; ADL, 0.6.

3Corn–SBM–rapeseed cake (RSC)-based basal diet of Exp. 2; analyzed concentration of nutrients of RSC, in % on DM basis: P, 1.24; Ca, 1.54; CP, 33.9; EE, 14.9; NDF, 23.0; ADF, 19.2; ADL, 6.8.

4Intrinsic plant phytase activity of basal diets was below the detection limit.

5Below the limit of quantification.

6Determined at pH 4.5 and 60 °C.

7Asp, Asn, and Glu, Gln, respectively, were detected together because the side groups of Asn and Gln are lost during acid hydrolysis (Fontaine, 2003).