Table 3.
Effects of increasing the inclusion level of phytase on the concentrations of different inositol phosphate (InsP) isomers and myo-inositol in ileal digesta and feces, µmol/g DM (Exp. 1)1
| BD2 | ||||||
|---|---|---|---|---|---|---|
| Phytase: | 0 | 750 | 1.500 | 3,000 | Pooled SEM | P-value |
| Ileal digesta | ||||||
| Ins(1,2)P2 | n.d.3 | 1.8 | 1.8 | 1.7 | 0.4 | 0.999 |
| InsP3x4 | n.d. | 2.9b | 1.8a | 1.7a | 0.3 | 0.009 |
| Ins(1,5,6)P3 | <LOQ5 | <LOQ | 0.2 | <LOQ | . | . |
| Ins(1,2,3,4)P4 | 0.2 | n.d. | n.d. | n.d. | . | . |
| Ins(1,2,5,6)P4 | 0.4a | 8.7c | 6.0b | 5.0b | 0.8 | <0.001 |
| Ins(1,2,3,4,6)P5 | 0.6 | n.d. | n.d. | n.d. | . | . |
| Ins(1,2,3,4,5)P5 | 1.4b | 1.2b | 0.6a | 0.4a | 0.1 | <0.001 |
| Ins(1,2,4,5,6)P5 | 2.6c | 0.6b | 0.4a | 0.3a | 0.1 | <0.001 |
| InsP6 | 36.0c | 10.5b | 7.2a | 6.5a | 0.8 | <0.001 |
| Myo-inositol | 1.8a | 6.3b | 9.2c | 9.7c | 1.1 | <0.001 |
| Feces | ||||||
| Ins(1,2,5,6)P4 | n.d. | 0.3 | n.d. | 0.2 | 0.1 | 0.250 |
| Ins(1,2,3,4,5)P5 | n.d. | 0.2 | n.d. | n.d. | 0.1 | . |
| Ins(1,2,4,5,6)P5 | 0.4b | 0.3ab | n.d. | 0.2a | 0.1 | 0.046 |
| InsP6 | 3.3 | 2.5 | 1.5 | 2.6 | 0.7 | 0.178 |
| Myo-inositol | 1.2c | 0.9bc | 0.4a | 0.5ab | 0.2 | 0.014 |
1Data are given as LSMeans and pooled SEM (untransformed data); InsP not mentioned here were not detectable in ileal digesta and feces. InsP1 isomers were not well separated from co-eluting matrix components and, therefore, not precisely identified. However, concentrations of InsP1 isomers potential including other components were <1.5 µmol/g DM for all samples.
2Corn–soybean meal-based basal diet.
3n.d., not detectable in the majority of samples.
4At least one of the following isomers: Ins(1,2,6)P3, Ins(1,4,5)P3, Ins(2,4,5)P3.
5<LOQ, below limit of quantification in the majority of samples.
a–cWithin a row, means without a common superscript differ (P < 0.05).