Table 6.

Concentrations of inositol phosphate (InsP) isomers and myo-inositol in ileal digesta and feces of pigs fed corn–soybean meal- or corn–soybean meal–rapeseed cake-based diets without or with the supplementation of phytase, µmol/g DM (Exp. 2)¹

	BDSBM2		BDRSC3			P-value
Phytase:	0	1,500	0	1,500	SEM	Protein source	Phytase	Protein source × phytase
Ileal digesta
Ins(1,2)P2	n.d.4	5.0	n.d.	5.3	0.7	0.985	.	.
InsP3x5	n.d.	4.7	0.8	4.3	0.5	0.587	< 0.001	.
Ins(1,2,3,4)P4	0.3	n.d.	0.5	n.d.	0.1	< 0.001	.	.
Ins(1,2,5,6)P4	0.4	8.4	0.8	8.3	1.2	0.664	< 0.001	0.867
Ins(1,2,3,4,6)P5	0.5	n.d.	<LOQ6	n.d.	0.0	.	.	.
Ins(1,2,3,4,5)P5	1.2a	0.4c	1.0ab	0.8b	0.1	0.379	< 0.001	0.017
Ins(1,2,4,5,6)P5	3.0	0.2	2.7	0.3	0.1	0.944	< 0.001	0.050
InsP6	32.2	3.9	43.2	4.7	1.9	0.028	< 0.001	0.565
Myo-inositol	4.7	17.7	2.7	13.2	1.6	0.004	< 0.001	0.787
Feces
Ins(1,2,5,6)P4	0.5	n.d.	0.5	0.3	0.2	0.809	0.263	.
Ins(1,2,4,5,6)P5	0.7	n.d.	1.5	0.3	0.4	0.045	0.003	.
InsP67	1.3	1.3	1.8	1.3	0.4	0.430	0.364	0.875
Myo-inositol	0.3	0.5	0.6	<LOQ	0.1	0.187	0.324	.

¹Data are given as LSMeans and pooled SEM (untransformed data); InsP not mentioned here were not detectable. InsP₁ isomers were not well separated from co-eluting matrix components and, therefore, not precisely identified. However, concentrations of InsP₁ isomers potential including other components were < 1.5 µmol/g DM for all samples.

²Corn–soybean meal-based basal diet.

³Corn–soybean meal-rapeseed cake-based basal diet.

⁴n.d., not detectable in the majority of samples.

⁵At least one of the following isomers: Ins(1,2,6)P₃, Ins(1,4,5)P₃, Ins(2,4,5)P₃.

⁶<LOQ, below limit of quantification in the majority of samples.

⁷Number of observations for BD_SBM and BD_RSC without phytase supplementation were n = 5 and n = 4, respectively, and for BD_SBM and BD_RSC with phytase supplementation were n = 6, respectively.

^a–cMeans in a row not sharing a common superscript differ significantly (multiple t-tests in case of interaction) (P < 0.05).