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. 2020 Feb 12;43(4):1208–1220. doi: 10.3892/or.2020.7499

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Copyright: © Bai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — MiD49 induces pancreatic cancer (PC) cell apoptosis. (A) Cell apoptosis was determined by Annexin V/PI staining using flow cytometry in PC cells with different levels of MiD49 as indicated. (B) Levels of cytochrome c (Cyt c) in the cytoplasm (Cyto) and mitochondria (Mito) were determined by western blot analysis in PC cells with different levels of MiD49 as indicated. (C) Levels of cleaved caspase 3 and cleaved caspase 9 were determined by western blot analysis in PC cells with different levels of MiD49 as indicated. *P<0.05. MiD49, mitochondrial dynamics protein of 49 kDa; siMiD49, siRNA against MiD49; siCtrl, control siRNA; MiD49, expression vector encoding MiD49; EV, empty vector.