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. 2020 Feb 12;43(4):1208–1220. doi: 10.3892/or.2020.7499

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Copyright: © Bai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 8. — Downregulation of MiD49 is mediated by an elevated miR-424 level in pancreatic cancer (PC) cells. (A) The level of miR-424 was determined by qPCR analysis in 20-paired tumor and adjacent non-tumor tissues of PC. (B) Correlation between the mRNA expression levels of MiD49 and miR-424 in tumor tissues from 20 PC patients. (C and D) The expression of MiD49 was determined by qPCR and western blot analyses in SW1990 and AsPC-1 cells transfected with synthetic precursor of miR-424. (E and F) MTS cell viability and colony formation assays in SW1990 and AsPC-1 cells transfected with synthetic precursor of miR-424. (G and H) Scratch wound-healing and Transwell invasion assays in SW1990 and AsPC-1 cells transfected with synthetic precursor of miR-424. (I) The promoter methylation profile analysis of MiD49 using the online web portal UALCAN. MiD49, mitochondrial dynamics protein of 49 kDa. *P<0.05.