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. 2020 Feb 12;43(4):1208–1220. doi: 10.3892/or.2020.7499

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Copyright: © Bai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 9. — MiD49 suppresses pancreatic cancer (PC) growth and metastasis through decreased ROS production. (A) Left panel: Representative images of mitochondria in PC cells with different MiD49 levels as indicated. Scale bars, 1 µm. Right panel: proportions of PC cells with elongated, intermediate, and fragmented mitochondria were quantified. (B) The level of intracellular ROS was determined by flow cytometry in PC cells with different MiD49 levels as indicated. (C and D) MTS cell viability and colony formation assays in PANC-1 cells treated with 90 µM H₂O₂ or SW1990 cells treated with 30 mM N-acetyl-L-cysteine (NAC) for 12 h as indicated. (E and F) Scratch wound-healing and Transwell invasion assays in PANC-1 cells treated with 90 µM H₂O₂ or SW1990 cells treated with 30 mM NAC for 12 h as indicated. MiD49, mitochondrial dynamics protein of 49 kDa; ROS, reactive oxygen species; siRNA against MiD49; siCtrl, control siRNA; MiD49, expression vector encoding MiD49; EV, empty vector. *P<0.05.

Figure 9. — MiD49 suppresses pancreatic cancer (PC) growth and metastasis through decreased ROS production. (A) Left panel: Representative images of mitochondria in PC cells with different MiD49 levels as indicated. Scale bars, 1 µm. Right panel: proportions of PC cells with elongated, intermediate, and fragmented mitochondria were quantified. (B) The level of intracellular ROS was determined by flow cytometry in PC cells with different MiD49 levels as indicated. (C and D) MTS cell viability and colony formation assays in PANC-1 cells treated with 90 µM H₂O₂ or SW1990 cells treated with 30 mM N-acetyl-L-cysteine (NAC) for 12 h as indicated. (E and F) Scratch wound-healing and Transwell invasion assays in PANC-1 cells treated with 90 µM H₂O₂ or SW1990 cells treated with 30 mM NAC for 12 h as indicated. MiD49, mitochondrial dynamics protein of 49 kDa; ROS, reactive oxygen species; siRNA against MiD49; siCtrl, control siRNA; MiD49, expression vector encoding MiD49; EV, empty vector. *P<0.05.