Table II.
Primary antibodies used in the western blot and IHC analyses.
| Antibody | Company (cat. no.) | Working concentration dilutions |
|---|---|---|
| MiD49 | Proteintech (16413-1-AP) | WB: 1/8 IHC: 1/150 |
| Caspase-9 | Proteintech (66169-1-Ig) | WB: 1/800 |
| Caspase-3 | Proteintech (25546-1-AP) | WB: 1/800 |
| Cytochrome c | Proteintech (10993-1-AP) | WB: 1/1,000 |
| COX IV | Abgent (#AP9153a) | WB: 1/1,000 |
| E-cadherin | Proteintech (20874-1-AP) | WB: 1/1,000 |
| N-cadherin | Cell Signaling (13116) | WB: 1/1,000 |
| Vimentin | Proteintech (10366-1-AP) | WB: 1/1,000 |
| ZO-1 | Proteintech (21773-1-AP) | WB: 1/1,000 |
| Ki-67 | MAB (MAB-0129) | IHC:1/200 |
| β-actin | Beijing TDY (TDY051F) | WB: 1/3,000 |
IHC, immunohistochemical; WB, western blotting; MiD49, mitochondrial dynamics protein of 49 kDa.