Table 1.
Parameters of the native inducible systems.
| Inducible system | Inducer | Dynamic range, in -folda | Kmb | hc |
|---|---|---|---|---|
| OapR/PH16_RS01330 | β-Alanine | 8.0 ± 0.4 | 201 ± 24 µM | 0.75 ± 0.05 |
| FdsR/PH16_RS03165 | Formate | 4.5 ± 0.2 | 130 ± 7 µM | 1.05 ± 0.04 |
| XdhR/PH16_RS05055 | Xanthine | 16.0 ± 1.0 | 13.0 ± 1.4 µM | 1.03 ± 0.10 |
| PhgR/PH16_RS05530 | Phenylglyoxylate | 144.8 ± 106.5 | 595 ± 133 µM | 0.96 ± 0.13 |
| NahR/PH16_RS08125 | Salicylate | 650.8 ± 317.4 | 2.12 ± 0.49 µM | 0.66 ± 0.08 |
| BenM/PH16_RS09790 | Benzoate | 74.1 ± 10.7 | 12.6 ± 0.8 µM | 1.64 ± 0.14 |
| TtdR/PH16_RS10665 | Tartrate | 370.6 ± 232.2 | 61.5 ± 22.9 mM | 0.80 ± 0.09 |
| SauR/PH16_RS13695 | Sulfonatoacetate | 395.4 ± 193.7 | 7.3 ± 4.9 mM | 0.57 ± 0.06 |
| KynR/PH16_RS14030 | L-kynurenine | 26.9 ± 2.1 | 2.64 ± 0.18 µM | 0.98 ± 0.05 |
| HpdR/PH16_RS18295 | 3-Hydroxypropanoate | 25.2 ± 1.7 | 13.2 ± 1.9 µM | 0.64 ± 0.03 |
| PhhR/PH16_RS18365 | L-phenylalanine | 9.0 ± 0.8 | 16.9 ± 2.2 µM | 0.59 ± 0.04 |
| GabR/PH16_RS23655 | GABA | ND | ND | ND |
| HpdA/PH16_RS24175 | L-tyrosine | 38.3 ± 5.0 | 9.9 ± 2.2 µM | 0.72 ± 0.07 |
| BadR/PH16_RS27200 | Cyclohexanecarboxylate | 11.4 ± 10.9 | 43.8 ± 13.0 µM | 1.11 ± 0.28 |
| PcaQ/PH16_RS30145 | 3,4-Dihydroxybenzoate | 77.2 ± 29.8 | 8.63 ± 0.40 µM | 0.98 ± 0.03 |
| AcoR/PH16_RS19445 | Acetoin | 11.1 ± 1.6 | 1.23 ± 0.07 µM | 1.36 ± 0.10 |
Data are mean ± SD, n = 3. Source data are provided as a Source Data file. ND - not determined.
aDynamic range is defined as the -fold increase in fluorescence calculated by dividing the maximum level of fluorescence output by the basal level of fluorescence output.
bKm represents the inducer concentration at which the half-maximal activation of the inducible system is achieved.
ch—Hill coefficient.