Table 1.

Parameters of the native inducible systems.

Inducible system	Inducer	Dynamic range, in -folda	Kmb	hc
OapR/PH16_RS01330	β-Alanine	8.0 ± 0.4	201 ± 24 µM	0.75 ± 0.05
FdsR/PH16_RS03165	Formate	4.5 ± 0.2	130 ± 7 µM	1.05 ± 0.04
XdhR/PH16_RS05055	Xanthine	16.0 ± 1.0	13.0 ± 1.4 µM	1.03 ± 0.10
PhgR/PH16_RS05530	Phenylglyoxylate	144.8 ± 106.5	595 ± 133 µM	0.96 ± 0.13
NahR/PH16_RS08125	Salicylate	650.8 ± 317.4	2.12 ± 0.49 µM	0.66 ± 0.08
BenM/PH16_RS09790	Benzoate	74.1 ± 10.7	12.6 ± 0.8 µM	1.64 ± 0.14
TtdR/PH16_RS10665	Tartrate	370.6 ± 232.2	61.5 ± 22.9 mM	0.80 ± 0.09
SauR/PH16_RS13695	Sulfonatoacetate	395.4 ± 193.7	7.3 ± 4.9 mM	0.57 ± 0.06
KynR/PH16_RS14030	L-kynurenine	26.9 ± 2.1	2.64 ± 0.18 µM	0.98 ± 0.05
HpdR/PH16_RS18295	3-Hydroxypropanoate	25.2 ± 1.7	13.2 ± 1.9 µM	0.64 ± 0.03
PhhR/PH16_RS18365	L-phenylalanine	9.0 ± 0.8	16.9 ± 2.2 µM	0.59 ± 0.04
GabR/PH16_RS23655	GABA	ND	ND	ND
HpdA/PH16_RS24175	L-tyrosine	38.3 ± 5.0	9.9 ± 2.2 µM	0.72 ± 0.07
BadR/PH16_RS27200	Cyclohexanecarboxylate	11.4 ± 10.9	43.8 ± 13.0 µM	1.11 ± 0.28
PcaQ/PH16_RS30145	3,4-Dihydroxybenzoate	77.2 ± 29.8	8.63 ± 0.40 µM	0.98 ± 0.03
AcoR/PH16_RS19445	Acetoin	11.1 ± 1.6	1.23 ± 0.07 µM	1.36 ± 0.10

Data are mean ± SD, n = 3. Source data are provided as a Source Data file. ND - not determined.

^aDynamic range is defined as the -fold increase in fluorescence calculated by dividing the maximum level of fluorescence output by the basal level of fluorescence output.

^bK_m represents the inducer concentration at which the half-maximal activation of the inducible system is achieved.

^ch—Hill coefficient.