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. 2020 Mar 5;10:4112. doi: 10.1038/s41598-020-60952-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Growth curves of cannabidiol (CBD) in combination with bacitracin (BAC). Bacterial density (BCA: Background corrected absorption) was measured using an oCelloScope for 24 hours; (a) Methicillin-resistant Staphylococcus aureus USA300 FPR 3757, (b) Enterococcus faecalis (13-327129), (c) Listeria monocytogenes EGD, (d) Methicillin-resistant Staphylococcus epidermidis. (e) Time-kill assay showing the effect of CBD and BAC in monotherapy and combination in MRSA USA300 FPR3757. Viability was monitored by OD₆₀₀ readings and CFU/mL determinations. Growth experiments were repeated at least three times and the time-kill assay was repeated twice with similar results.