Table 2.
In vitro antimicrobial activity of actinobacteria isolated from forest ecosystems of Assam, India by spot inoculation method.
| Sl. No. | Isolate code | *Zone of inhibition (mm) | |||
|---|---|---|---|---|---|
| Bacterial test microorganisms | Fungal test microorganism | ||||
| S. aureus MTCC 96 | MRSA ATCC 43300 | E. coli MTCC 40 | C. albicans MTCC 227 | ||
| 1. | PB-9 | 11a ± 0.5 | ND | 12ab ± 1.2 | ND |
| 2. | PB-10 | ND | ND | ND | 13abcdef ± 1 |
| 3. | PB-15 | 26l ± 1 | 14efgh ± 2 | 18hi ± 1.5 | 20n°p ± 1 |
| 4. | PB-17 | 31mn ± 1 | ND | ND | 11a ± 1 |
| 5. | PB-19 | 14 cdefg ± 1.5 | 11ab ± 1.6 | ND | 13abcdef ± 2 |
| 6. | PB-20 | ND | ND | 12ab ± 0.7 | ND |
| 7. | PB-21 | 32n ± 0.7 | ND | 55x ± 1.5 | 20nop ± 1 |
| 8. | PB-22 | ND | ND | ND | 15efghij ± 1.5 |
| 9. | PB-25 | 20ij ± 1.2 | 11abc ± 1.7 | ND | 15cdefghi ± 1.5 |
| 10. | PB-26 | 13abc ± 1 | ND | 15ef ± 1.7 | ND |
| 11. | PB-27 | ND | ND | 30tu ± 2 | ND |
| 12. | PB-28 | 19hi ± 2 | 20° ± 0.7 | 35v ± 0.5 | 18lmn ± 2.7 |
| 13. | PB-31 | ND | ND | 46w ± 3 | ND |
| 14. | PB-32 | ND | ND | 20ij ± 0.7 | 12abc ± 1 |
| 15. | PB-33 | 18h ± 0.7 | 17jkl ± 1.3 | 20ij ± 1.2 | 12abcd ± 1.2 |
| 16. | PB-39 | 14cde±1 | 12abcd ± 2 | 28qrs ± 0.5 | 14bcdefgh ± 1.7 |
| 17. | PB-43 | 26l ± 1.5 | 18lmn ± 1 | 27pqrs ± 2 | 12ab ± 0.7 |
| 18. | PB-46 | ND | ND | ND | 36x ± 1 |
| 19. | PB-47 | 14bcd ± 1.5 | ND | ND | 16ghijkl ± 1.5 |
| 20. | PB-48 | 15defg ± 1 | 19no ± 1.2 | 30tu ± 1.5 | 40z ± 1.7 |
| 21. | PB-50 | 38p ± 0.7 | ND | 15ef ± 1 | ND |
| 22. | PB-51 | 14cdef ± 1.2 | 15ghij ± 2 | 28rs ± 1 | 17hijklm ± 1.4 |
| 23. | PB-52 | 36o ± 0.8 | 30r ± 0.8 | 31 u ± 1.2 | 27u ± 0.4 |
| 24. | PB-54 | 15cdefg ± 1.2 | 12abcd ± 1.6 | 36 v ± 1 | 14bcdefg ± 2 |
| 25. | PB-55 | ND | ND | 13abc ± 1.3 | 15fghijk ± 1 |
| 26. | PB-56 | 15cdefg ± 2.5 | ND | 14cdef ± 0.5 | 18lmn ± 1 |
| 27. | PB-64 | 26l ± 1.2 | 25p ± 0.5 | 14bcde ± 0.5 | 17ijklm±1.5 |
| 28. | PB-65 | 41q ± 1 | 28q ± 0.7 | 56x±0.8 | 15fghijk ± 1.2 |
| 29. | PB-66 | ND | ND | ND | 37xy ± 1 |
| 30. | PB-68 | 60s ± 0.5 | 13def ± 1.7 | 20ij ± 1 | 18lmn ± 2.7 |
| 31. | PB-70 | 31n ± 1.5 | 30r ± 1.5 | 55x ± 0.7 | 14bcdef ± 2 |
| 32. | PB-75 | 19hi ± 1.7 | ND | 15def ± 1.5 | 19mno ± 1.7 |
| 33. | PB-76 | 16fg ± 1 | 20o ± 1.2 | 13abcde ± 1.7 | 13abcde ± 1.3 |
| 34. | PB-79 | 12ab ± 1 | ND | 25nop ± 2 | ND |
| 35. | PB-81 | 16efg ± 0.7 | 31r ± 1.5 | 19hij ± 1.2 | ND |
| 36. | PB-82 | ND | ND | 16fg ± 1 | ND |
| 37. | PB-83 | 14cdef ± 1.2 | 10a ± 2 | 27opqr ± 1.5 | 17jklm ± 1 |
| 38. | PB-85 | 21jk ± 0.7 | 13bcde ± 1.3 | 46w ± 1.2 | 12abc ± 1 |
| 39. | PB-86 | 19hi ± 1 | 30r ± 1.2 | 30tu ± 2 | 22pqr ± 0.8 |
| 40. | Kz-2 | ND | 13cde ± 1 | 14cdef ± 1.5 | 15fghijk ± 0.5 |
| 41. | Kz-10 | ND | 17klm ± 1.2 | 12a ± 1.3 | 18lmn ± 0.7 |
| 42. | Kz-11 | ND | 15ghij | 16fg ± 0.7 | 17klm ± 1 |
| 43. | Kz-12 | 25l ± 1.5 | 13defg ± 1.5 | 35v ± 0.7 | ND |
| 44. | Kz-13 | 15defg ± 1 | 14efgh ± 1 | 21jk ± 0.7 | 18lmn ± 2 |
| 45. | Kz-14 | ND | 16ijkl ± 1.2 | 26nopq ± 0.7 | 23rs ± 1 |
| 46. | Kz-18 | ND | 16ijkl ± 1.5 | 31tu ± 0.7 | 20nopq ± 1.2 |
| 47. | Kz-21 | ND | 19mno ± 0.5 | 26nopq ± 0.7 | 21opqr ± 1.5 |
| 48. | Kz-23 | 16g ± 1.2 | ND | 20ij | 23qrs ± 0.8 |
| 49. | Kz-24 | 42q ± 2 | 56t ± 1 | 26nopq ± 0.5 | 52Ψ ± 1.8 |
| 50. | Kz-27 | ND | ND | 20ij ± 1 | 25tu ± 1.2 |
| 51. | Kz-28 | 29m ± 0.5 | 25p ± 1 | 24lmn ± 2 | 31v ± 0.7 |
| 52. | Kz-29 | 31mn ± 0.5 | 25p ± 1.5 | 27opqr ± 2 | ND |
| 53. | Kz-31 | ND | ND | ND | 17jklm ± 0.7 |
| 54. | Kz-32 | 70t ± 1.3 | 12abcd ± 0.7 | ND | 30v ± 1.5 |
| 55. | Kz-36 | 51r ± 1 | ND | 29st ± 0.7 | 34w ± 1 |
| 56. | Kz-38 | ND | 15ghij ± 1 | ND | 15defghij ± 0.5 |
| 57. | Kz-41 | 31n ± 1.2 | 16hijk ± 1 | 20ij ± 0.2 | 21opqr ± 1 |
| 58. | Kz-42 | ND | ND | ND | 12ab ± 1.7 |
| 59. | Kz-44 | 20ij ± 1 | 14efghi ± 1.5 | 20ij ± 0.7 | 14cdefghi ± 1.5 |
| 60. | Kz-47 | 20ij ± 2 | 10a | 20ij ± 1 | 40yz ± 1.2 |
| 61. | Kz-49 | 15defg ± 1 | 15ghij ± 1.2 | ND | ND |
| 62. | Kz-52 | 13abc ± 2 | 25p ± 0.5 | ND | 15efghij ± 1.5 |
| 63. | Kz-55 | 31mn ± 0.7 | 16hijk ± 0.8 | 20hij ± 1 | 17ghijkl ± 1.5 |
| 64. | Kz-56 | 21jk ± 1 | 15hijk ± 1.5 | 25mno ± 0.5 | ND |
| 65. | Kz-58 | ND | 16hijk ± 1 | 23lm ± 1.7 | ND |
| 66. | Kz-61 | ND | ND | 18hij ± 0.5 | ND |
| 67. | Kz-62 | 31n ± 1.5 | 37s ± 0.7 | ND | ND |
| 68. | Kz-66 | 19hi ± 1 | 12bcd | 19hij ± 2 | 20nop ± 1.7 |
| 69. | Kz-67 | 22k ± 0.5 | 14efgh ± 1.7 | 26opqr ± 1.2 | 19mno ± 1 |
| 70. | Kz-72 | ND | ND | ND | 21opqr ± 1 |
| 71. | Kz-73 | ND | ND | ND | 22pqrs ± 2 |
| 72. | Kz-74 | 25l ± 1.2 | 15fghij ± 1.7 | 23kl ± 0.8 | 26u ± 1.5 |
| 73. | Kz-75 | ND | ND | ND | 24st ± 1.2 |
| 74. | Kz-76 | 26l ± 0.8 | 12bcd ± 1 | 18gh ± 1 | ND |
| 75. | Kz-78 | 25l | 13cde ± 0.7 | 18gh ± 1.5 | 30v ± 2 |
| 76. | Kz-79 | ND | 15ghij ± 1.2 | 13abcd ± 1 | 17jklm ± 1.6 |
| 77. | Kz-80 | 21ij ± 1.8 | 38s ± 1.2 | 19hij ± 1 | 20nop ± 2 |
The average size of colony of the actinobacteria isolates in GLM agar were (7 ± 2) mm in diameter after 5–10 days of incubation at 28 °C.
*Zone of inhibition by spot inoculation method on GLM agar medium.
Zone of inhibition values are given as mean ± SD (n = 3). Values having different superscripts (a–z, Ψ) differ significantly (P < 0.05).
ND: not detectable.