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. 2020 Mar 5;10:4123. doi: 10.1038/s41598-020-60275-0

Figure 1.

Figure 1

Inducing the infiltration of CD4+ T cells into HSCs. (a) Method for generation of immunocompetent HSC. (b) 3D-reconstructed whole-mount image of HSCs showing 3D conformation of K14-positive epidermis and CD3-positive T cells with and without the epidermis (DAPI: blue). (c) Quantification of the number and penetration depth of infiltrated T cells in HSCs (µm). (d) Orthogonal segment of T cell-bearing HSCs with the centerline of their initial position on the gel surface as a reference (white dotted line) showing CD3-positive (green) T cells (DAPI: blue). (e) Quantification of the total number of cells that migrated upward (dermis) or downward (acellular gel).