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. 2020 Mar 5;10:4105. doi: 10.1038/s41598-020-60871-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Photomicrograph of thrombi in the portal vein (*; Victoria blue and haematoxylin-eosin stain [VB-HE]) with the primary tumour features (small windows in the right upper corner; VB-HE, x 50) in hepatocellular carcinomas surgically removed in patients (A to F). Portal-vein thrombi showing no evidence of viable tumour in Patients (A,C,F) (VB-HE, x 40), or containing residues of tumour cells in patients (B,D,E) with varying degrees of degeneration (VB-HE, x 100).