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. 2020 Mar 5;10:4158. doi: 10.1038/s41598-020-60984-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GPR43 suppresses insulin signalling in the adipose tissue, but not in the muscle or liver. Representative images of immunoreactive bands images (a,c) and its intensities representing (b,d) the insulin-stimulated Akt phosphorylation of Ser473 and total Akt in the adipose tissue, muscle and liver of WT mice (n = 4) and Gpr43−/− mice (n = 3) that had fasted for 6 h. Data are presented as the mean ± SEM, and were analysed with Tukey’s test. Different letters next to bars indicate a significant difference (P < 0.05).