Fig. 2. Effect of TRPC6-blockage on O₃-induced airway inflammation in mice.

WT mice were exposed to O₃ (1 ppm) for 3 h every other day (day 1, 3, 5). Mice received PBS or SAR7334 by oral gavage 4 h before exposure. The mice were anesthetized 24 h after the last exposure. a–c Total white blood cell counts (Total WBC), macrophage counts (Mac), neutrophil counts (Neu), lymphocyte counts (Lym), eosinophil counts (Eos) (a), total protein content (b) and the release of inflammatory mediators IL-6, IL-8, TNF-α (c) in BAL fluid of different groups were compared. d Inflammation scores in air control and O₃-exposed mice. e Representative histological sections of mouse lungs (H&E staining) after exposure to O₃ or air. Black arrows: inflammatory changes. Scale bar: 100 μm. Results are presented as mean ± SEM, n = 8. *P < 0.05 or **P < 0.01 compared with PBS + Air group, ^##P < 0.01 compared with PBS + O₃ group, ^&&P < 0.01 compared with SAR (5 μM) + O₃ group.