Fig. 7. Role of MAPK signal pathway in oxidative stress-induced inflammatory response in bronchial epithelial cells.

a–c Western blot analysis of phosphorylation protein expression of ERK (a), p38 (b) and JNK (c) after 16HBE cells were stimulated with O₃ (100 ppb) or H₂O₂ (100 μM) for 0, 15, 30, 60, 120 min. ^**P < 0.01 compared with 0-min group. d–f After pretreatment with or without PD98059 (1, 10, 25 μM) (d), SB203580 (1, 10, 25 μM) (e) or SP600125 (1, 10, 25 μM) (f) for 1 h, 16HBE cells were stimulated with O₃ (100 ppb) for 6 h followed by continuing culture for another 24 h or stimulated with H₂O₂ (100 μM) for 24 h. Release levels of IL-6 and IL-8 were detected. Data represent the mean ± SEM, n = 5. ^**P < 0.01 compared with Control group, ^##P < 0.01 compared with H₂O₂ or O₃ group.