Fig. 8. Role of TRPC6 in oxidative stress-induced activation of MAPK signal pathway.

a, b After NC or shTRPC6 infection, 16HBE cells were exposed to O₃ (100 ppb) for 30 min (a) or H₂O₂ (100 μM) for 15 min (b) respectively. Phosphorylation levels of ERK, p38, JNK were analyzed by western blot. Data represent the mean ± SEM, n = 5. ^**P < 0.01 compared with Control group, ^##P < 0.01 compared with O₃ or H₂O₂ group, ^&&P < 0.01 compared with NC + O₃ or NC + H₂O₂ group. c 16HBE cells were loaded with the Ca²⁺ indicator Fluo-4/AM (5 μM) in Hanks’ solution for 30 min to measure [Ca²⁺]_i. The [Ca²⁺]_i in the cells was expressed as a pseudo-ratio value of the relative fluorescence intensity (F/F₀) (Left). Bar graph shows the mean peak value of F/F₀ traces (Peak of F/F₀) of the experiments (Right). After pretreatment with or without PD98059 (25 μM), SB203580 (25 μM) or SP600125 (25 μM) for 1 h, 16HBE cells were stimulated with H₂O₂ (100 μM). **P < 0.01 compared with HBSS control group. Each trace represents the mean from three independent experiments that were derived from 20 to 40 cells in each single experiment. NC: Negative Control, shT6: shRNA TRPC6.