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. 2020 Mar 5;10:4142. doi: 10.1038/s41598-020-60280-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Different percentages of nuclear RORγt positivity. Panel (A) shows the diffuse brownish in cytoplasm but not in nuclear of thyroid carcinoma cells. Panel (B) evidence that major of nuclei of thyroid cancer cells are negative for RORγt expression (upper right panel). Lower right panel shows a detail of focus of nuclei positive for RORγt. Panel C represent a tissue spot in which all nuclei of thyroid cancer cells are positive for RORγt. Black arrows point to nuclei positive for RORγt. Black arrow head point to nucleai negative for RORγt. Stromal components like collagen, vessels and myocytes are also not expected to express nuclear RORγt, giving us a negative control for each spot analyzed.