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. 2020 Mar 5;10:4142. doi: 10.1038/s41598-020-60280-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemistry of interleukins. (A) IL-1β presented a diffuse, homogeneous and cytoplasmic pattern of expression in thyroid cancer cells. (B) IL-10 was seen as strong brownish expression in both nuclei and cytoplasm of malignant cells. (C) IL-17A presented a focal cytoplasmic expression among thyroid cancer cells. (D) IL-23 expression was observed in cytoplasm of malignant cells. All panels evidenced that stromal structures (eg. collagen, colloid and vessels) lack expression of immune markers.