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. 2020 Mar 5;11:1203. doi: 10.1038/s41467-020-15017-1

Fig. 6. Regulation of the methionine sensing by structural elements of the T-box riboswitch.

Fig. 6

a Secondary structure diagram of the regulatory element (RE; T-box riboswitch) used in this study. The four introduced mutations to the T-box are shown in red and with numbers (1–4). Highly conserved domains in T-box riboswitches are indicated. b Single-cell fluorescence measurements by flow cytometry, in the met promoter-driven expression of GFP in the L. lactis Pmet-gfp (WT) and each of the four mutants of the regulatory element in the met promoter (1–4), grown in CDM supplemented with increasing concentrations of methionine (0.025–10 mM; red to blue). 10,000 ungated events for each sample are shown. Source data are provided as a Source Data file. c Snapshots of single-cell fluorescence microscopy in all the L. lactis Pmet-gfp strains, grown in standard CDM (0.27 mM methionine). Scale bar, 15 µm.