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. 2020 Mar 5;10:4166. doi: 10.1038/s41598-020-60954-y

Figure 3.

Figure 3

Competitive binding assay of NBD-Bu (10 µM), pre-incubated with 100 µM BSA, with increasing concentration of (a) ibuprofen and (b) warfarin in PBS (pH 7.4); (inset: the intensity at 535 nm with increasing site-marker concentration) (c,d) molecular docking picture shows the binding interaction of NBD-Bu with BSA. The molecular docking result was analyzed by PyMOL (v2.1.1) and UCSF Chimera (v1.13, https://www.cgl.ucsf.edu/chimera/) for visualization and understanding.