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. 2020 Mar 5;11:1195. doi: 10.1038/s41467-020-14764-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The non-diabetic human fibroblast-hiPSC line C1.2 (Supplementary Data 1) was cultured in parallel in either primed, conventional E8 (PRIMED; P) or LIF-3i/MEF (NAÏVE; N) conditions prior to parallel injections into sibling NOG mice (5 × 10⁶ cells/site) for teratoma assays. Paraffin sections of 8 week-old N vs. P teratomas were evaluated and individual microscopic sections quantified by (a, b) H&E staining (cartilage (cart); neural rosettes (NR); retinal pigmented epithelium (RPE) (Scale Bar = 500 μm), or (c, d) Immunofluorescence (IF) staining (Scale Bar = 50 μm). Shown are individual tissue section measurements from at least 3 independent teratoma experiments quantified for organoid structures and markers of endodermal (Cytokeratin 8⁺ (CK8); gut/glandular structures), mesodermal (NG2+ chondroblasts), and ectodermal (SOX2⁺ neural rosettes) lineages along with the proliferation marker Ki-67. **p < 0.01; ***p < 0.001 (Mann-Whitney tests).